Genetic diversity of cytochrome P450 3A with different metabolic activity in domestic cats

The Journal of Veterinary Medical Science

Tomoko AMANO¹  Souta SUGIYAMA²  Yasuhiro UNO³ 

[1] College of Agriculture, Food and Environment Sciences, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan;School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan;Shin Nippon Biomedical Laboratories, Ltd., Kainan, Wakayama 642-0017, Japan

关键词: cytochrome P450;    domestic cat;    polymorphism;    xenobiotic;   

DOI  :  10.1292/jvms.18-0692

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Knowledge on genetic polymorphisms of metabolising enzymes including cytochrome P450 (CYP) is very limited in cats. We investigated polymorphisms in CYP3A131, one of the major CYP isoforms in the feline liver and small intestine. Eight non-synonymous variants and one synonymous variant of feline CYP3A131 were identified in 29 cats. A major non-synonymous type was not observed. Metabolic parameters (Km and Vmax) of dibenzylfluorescein hydroxylation were ranged within about 2 times for the identified non-synonymous variants by using a heterologous coexpression system of CYP3A131 and feline cytochrome P450 reductase in Escherichia coli. The results confirmed the polymorphic nature of CYP3A131 as a basis for effective application of medicines and prevention of adverse reactions in the treatment of domestic cats.

【 授权许可】

Unknown