Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process

Ciencia e Investigación Agraria

Andrés Quiroz¹  Ana Mutis¹  Manuel Chacón-Fuentes²  Ivette Seguel³  Alejandro Urzúa⁴  Leonardo Bardehle⁵ 

[1] Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Av. Francisco Salazar 01145, Casilla 54-D, Temuco, Chile;Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Av. Francisco Salazar 01145, Temuco, Chile.;Instituto de Investigaciones Agropecuarias, Centro Regional de Investigación Carillanca, Temuco, Chile.;Laboratorio de Química Ecológica, Departamento de Ciencias del Ambiente, Universidad de Santiago de Chile, Av. Bernardo O Higgins 3363, Santiago, Chile.;Scientific and Technological Bioresources Nucleus (BIOREN), Universidad de La Frontera, Casilla 54-D, Temuco, Chile.

关键词: Kaempferol;    cultivated;    enzyme activity;    naringenin;    wild;   

DOI  :  10.7764/rcia.v46i1.1955

学科分类：农业科学（综合）

来源: Pontificia Universidad Catolica de Chile

【 摘 要 】

Flavonoid biosynthesis may be affected by plant domestication, with flavonoid production being reduced in proportion to the degree of domestication. In this context, kaempferol (3,4´,5,7-tetrahydroxyflavone) has been identified in the leaves of wild and cultivatedUgni molinae , a berry endemic to Chile. The biosynthetic pathway of kaempferol production begins with naringenin (4´,5,7-trihydroxyflavanone), which is converted to dihydrokaempferol (3,4´,5,7-tetrahydroxyflavanone), catalyzed by flavanone 3ß-hydroxylase (FHT) and then converted to kaempferol by a bifunctional enzyme called flavonol synthase (FLS). Therefore, our study aims to evaluate how FLS activity is affected in murtilla plants that are subjected to the domestication process. Kaempferol was quantified from methanolic extracts of leaf samples collected from both cultivated and wildU. molinaeplants using high-performance liquid chromatography, and enzyme extraction was performed to determine FLS activity. The results showed that kaempferol concentration in wild plants from the Soloyo (0.14 μg g -1 ), Mehuín (0.18 μg g -1 ) and Queule (0.25 μg g -1 ) sampling areas was higher than in their cultivated counterparts. Our data are consistent with the FLS activity detected in samples obtained from Manzanal Alto (134.79 pKatal, Soloyo (96.48 pKatal), and Mehuín (119.97 pKatal). These samples also exhibited higher enzymatic activity than their cultivated counterparts. Together, these data suggest that FLS activity is negatively affected by the domestication process.

【 授权许可】

Unknown