Survey on pathogenic Escherichia coli and Salmonella spp. in captive cockatiels (Nymphicus hollandicus)

Revista de microbiologia

Pontes, Patricia Silveira de¹  Coutinho, Selene Dall Acqua² 

[1] Universidade Paulista, São Paulo, Brazil;Universidade de São Paulo, São Paulo, Brazil

关键词: Escherichia coli;    Salmonella sp;    Cockatiel;    Nymphicus hollandicus;    Antimicrobial resistance.;   

DOI  :  10.1016/j.bjm.2018.05.003

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

We surveyed healthy captive cockatiels (Nymphicus hollandicus) for Escherichia coli and Salmonella spp. Cloacal swabs were collected from 94 cockatiels kept in commercial breeders, private residencies and pet shops in the cities of São Paulo/SP and Niterói/RJ (Brazil). Three strains of E. coli from each individual were tested for the presence of ExPEC-, APEC- and DEC-related genes. We evaluated the blaTEM, blaSHV, blaOXA, blaCMY, blaCTX-M, tetA, tetB, aadA, aphA, strAB, sul1, sul2, sul3, qnrA, qnrD, qnrB, qnrS, oqxAB, aac (6)′-Ib-cr, qepA resistance genes and markers for plasmid incompatibility groups. Salmonella spp. was not detected. E. coli was isolated in 10% of the animals (9/94). Four APEC genes (ironN, ompT, iss and hlyF) were detected in two strains (2/27–7%), and iss (1/27–4%) in one isolate. The highest resistance rates were observed with amoxicillin (22/27–82%), ampicillin (21/27–79%), streptomycin (18/27–67%), tetracycline (11/27–41%). Multiresistance was verified in 59% (16/27) of the isolates. We detected strAB, bla TEM, tetA, tetB, aadA, aphaA, sul1, sul2, sul3 resistance genes and plasmid Inc groups in 20 (74%) of the strains. E. coli isolated from these cockatiels are of epidemiological importance, since these pets could transmit pathogenic and multiresistant microorganisms to humans and other animals.

【 授权许可】

CC BY-NC