Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance

Revista de microbiologia

Bassi, Ana Paula Guarnieri¹  Antonangelo, Ana Teresa Burlamaqui Faraco²  Reis, Vanda Renata³ 

[1] Genotyping Laboratório de Biotecnologia Ltda, Botucatu, Brazil;Universidade Estadual Paulista, Botucatu, Brazil;Universidade Federal de São Carlos, Araras, Brazil

关键词: Fermentation;    Stresses;    Yeasts;    Microsatellite;    Contaminants.;   

DOI  :  10.1016/j.bjm.2016.09.017

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cerevisiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive.

【 授权许可】

CC BY-NC