Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach

Revista de microbiologia

Shabbiri, Khadija¹  Adnan, Ahmad² 

[1] GC University, Lahore, Pakistan;University of the Punjab, Lahore, Pakistan

关键词: Brevibacterium linens;    Protease;    Solid-state fermentation;    Plackett-Burman Design;    Central Composite Design (CCD).;   

DOI  :  10.1590/S1517-83822012000300031

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.

【 授权许可】

CC BY-NC