Optimization of moistening solution concentration on xylanase activity in solid state fermentation from oil palm empty fruit bunches

2nd International Conference on Biomass: Toward Sustainable Biomass Utilization for Industrial and Energy Applications

生物科学;工业技术;能源学

Mardawati, Efri^1 ; Parlan^2 ; Rialita, Tita^2 ; Nurhadi, Bambang^2

Departement of Agro-Industrial Technology, Faculty of Agro-industrial Technology, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21, Jatinangor, Sumedang

40600, Indonesia^1

Departement of Food Industrial Technology, Faculty of Agro-industrial Technology, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21, Jatinangor, Sumedang

40600, Indonesia^2

关键词: Ammonium sulphate concentration;    Oil palm empty fruit bunch;    Oil palm empty fruit bunch (OPEFB);    Optimum concentration;    Potassium dihydrogen phosphate;    Response surface method;    Solid-state fermentation;    Solution concentration;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/141/1/012018/pdf
DOI  :  10.1088/1755-1315/141/1/012018

来源: IOP

【 摘 要 】

Xylanase is an enzyme used in the industrial world, including food industry. Xylanase can be utilized as a 1,4-β-xylosidic endo-hydrolysis catalyst of xylanase, a hemicellulose component for obtaining a xylose monomer. This study aims to determine the optimum concentration of the fermentation medium using Response Surface Method (RSM) in the production of xylanase enzyme from oil palm empty fruit bunches (OPEFB) through solid state fermentation process. The variables varied in this study used factor A (ammonium sulphate concentration 1.0-2.0 g/L), B (concentration of potassium dihydrogen phosphate 1.5-2.5 g/L) and C (urea concentration 0.2 - 0.5 g/L). The data was analysed by using Design Expert version 10.0.1.0 especially CCD with total 17 running including 3 times replicated of canter point. Trichoderma viride was used for the process production of xylanase enzyme. The ratio between substrate and moistening solution used was 0.63 g / mL with temperature of 32.80C, 60 h incubation time. The analysis of enzyme activity was done by DNS method with 1% xylan as substrate. Analysis of protein content in enzyme was done by Bradford method. The optimum of moistening solution concentration in this fermentation was obtained. They are, the ammonium sulphate concentration of 1.5 g/L, potassium dihydrogen phosphate 2.0 g/L and urea 0.35 g/L with activity of 684.70 U/mL, specific activity enzyme xylanase 6261.58 U/mg, protein content 0.1093 U/mg, the model was validated using experiment design with perfect reliability value 0.96.