Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor

Brazilian Archives of Biology and Technology

Seema Patel¹  Damini Kothari¹  Rishikesh Shukla¹  Debasish Das¹  Arun Goyal¹ 

[1] ,Indian Institute of Technology Guwahati Department of Biotechnology Assam,India

关键词: Pediococcus pentosaceus;    dextran;    Plackett-Burman Design;    Central Composite Design;    Response Surface Methodology;   

DOI  :  10.1590/S1516-89132011000600007

来源: SciELO

【 摘 要 】

The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type. Response surface methodology was carried out for further enhancement of dextran production. To enhance dextran production by the mutant SPAm, Plackett-Burman Design and a 2² full factorial Central Composite Design was employed. After response optimization, the optimum concentration of sucrose and yeast extract was 5.115% (w/v) and 0.635% (w/v), respectively. The experimental values of dextran 36.0 mg/ml at flask level and 35.0 mg/ml at bioreactor level were in good agreement with the predicted value of 40.8 mg/ml. The increase in dextran production by the mutant SPAm using the optimized medium was 3 fold higher as compared to unoptimized medium.

【 授权许可】

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