Isolation of an Egg-Adapted Influenza A(H3N2) Virus without Amino Acid Substitutions at the Antigenic Sites of Its Hemagglutinin

Japanese journal of infectious diseases

Kazuya Nakamura¹  Masayuki Shirakura²  Seiichiro Fujisaki³  Tomoko Kuwahara⁴  Emi Takashita⁵ 

[1] Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison;Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo;Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo;Influenza Virus Research Center, National Institute of Infectious Diseases;Saitama Institute of Public Health

关键词: influenza A(H3N2) virus;    egg adaptation;    hemagglutinin (HA);    neuraminidase (NA);    antigenicity;   

DOI  :  10.7883/yoken.JJID.2017.551

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

Antigenic changes in the hemagglutinin protein of recent A(H3N2) viruses often arise when these viruses adapt to their egg host. By serial egg passages of a cell-propagated virus, we successfully isolated an egg-adapted influenza A(H3N2) virus, A/Saitama/103/2014, without amino acid substitutions at the antigenic sites of its hemagglutinin protein but with multiple substitutions in its neuraminidase protein. Antigenic analysis of this egg-adapted A/Saitama/103/2014 virus indicated that its antigenicity did not differ from that of the World Health Organization prototype cell-propagated vaccine virus: A/Hong Kong/4801/2014. Our results suggest that this strategy may facilitate egg-based vaccine production without antigenic alterations in hemagglutinin by egg adaptation.

【 授权许可】

Unknown