Japanese journal of infectious diseases | |
Isolation of an Egg-Adapted Influenza A(H3N2) Virus without Amino Acid Substitutions at the Antigenic Sites of Its Hemagglutinin | |
Kazuya Nakamura1  Masayuki Shirakura2  Seiichiro Fujisaki3  Tomoko Kuwahara4  Emi Takashita5  | |
[1] Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison;Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo;Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo;Influenza Virus Research Center, National Institute of Infectious Diseases;Saitama Institute of Public Health | |
关键词: influenza A(H3N2) virus; egg adaptation; hemagglutinin (HA); neuraminidase (NA); antigenicity; | |
DOI : 10.7883/yoken.JJID.2017.551 | |
学科分类:传染病学 | |
来源: National Institute of Infectious Diseases | |
【 摘 要 】
Antigenic changes in the hemagglutinin protein of recent A(H3N2) viruses often arise when these viruses adapt to their egg host. By serial egg passages of a cell-propagated virus, we successfully isolated an egg-adapted influenza A(H3N2) virus, A/Saitama/103/2014, without amino acid substitutions at the antigenic sites of its hemagglutinin protein but with multiple substitutions in its neuraminidase protein. Antigenic analysis of this egg-adapted A/Saitama/103/2014 virus indicated that its antigenicity did not differ from that of the World Health Organization prototype cell-propagated vaccine virus: A/Hong Kong/4801/2014. Our results suggest that this strategy may facilitate egg-based vaccine production without antigenic alterations in hemagglutinin by egg adaptation.
【 授权许可】
Unknown
【 预 览 】
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