Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils

Revista de microbiologia

Aanniz, Tarik¹  Melloul, Marouane²  Ouadghiri, Mouna³ 

[1] Centre National pour la Recherche Scientifique et Technique, Rabat, Maroc;Ghent University, Gent, Belgium;Université Moulay Ismail, Meknès, Maroc

关键词: thermophilic bacteria;    hot springs;    diversity;    16S rRNA gene sequencing;    hydrolytic enzymes.;   

DOI  :  10.1590/S1517-838246220140219

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented by A. pallidus (4) and Aeribacillus sp. (2). In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.

【 授权许可】

CC BY-NC