Ethylenediaminetetraacetic Acid Inhibits Vibrio Vulnificus-Induced Dendritic Cell Apoptosis by Lowering [Ca2+]i

Neuro-signals

Yuzhang Zhu^2¹  Ping Qian^1²  Bo Hu^1³  Dongliang Shao^3⁴ 

[1] Department of Anesthesiology and Neonatal Intensive Care Unit, Jiaxing Maternity and Children Health Care Hospital,Jiaxing University, Jiaxing^3;Department of Immunopathology, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University^1;Department of Oncology, Second Affiliated Hospital of Jiaxing University^2;Department of Oncology, Tonghua Central Hospital, Tonghua, P.R.China^4

关键词: Vibrio vulnificus;    Dendritic cell;    EDTA;    [Ca2+]i;    STAT3;    m-Tor;    Bcl-2;   

DOI  :  10.1159/000489951

学科分类：神经科学

来源: S Karger AG

【 摘 要 】

Background/Aims Vibrio vulnificus (V. vulnificus) is a Gram-negative marine bacterium that can cause life-threatening primary septicemia, especially in the innate immune system. But how V. vulnificus affects and acts on dendritic cells (DC) is not well understood. The aim of the present study is to investigate [Ca2+]i change and the expression of the mTor-STAT3-Bcl-2 signaling pathway in V. vulnificus B2-induced DC apoptosis, and explore the protective effect of ethylenediaminetetraacetic acid (EDTA) against DC apoptosis in a V. vulnificus B2 and DC2.4 cell coculture infection model, using EDTA as an intervenient. Methods The apoptosis rate, [Ca2+]i, and the expression of STAT3, m-Tor and Bcl-2 were detected by cytometry, Fluo-8-AM and Western blotting respectively. Results The results demonstrated that EDTA inhibited the increase of [Ca2+]i, upregulated the expression of m-Tor-STAT3-Bcl-2 signaling pathway, and protected DC against V. vulnificus B2-induced apoptosis. Conclusions EDTA inhibits V. Vulnificus-induced DC apoptosis by lowering [Ca2+]i via m-Tor-STAT3-Bcl-2 signaling pathway.

【 授权许可】

CC BY-NC-ND