Skeletal muscle contractions stimulate cGMP formation and attenuate vascular smooth muscle myosin phosphorylation via nitric oxide

FEBS Letters

Chang, Wen-Jinn¹  Kamm, Kristine E.¹  Sarelius, Ingrid²  Stull, James T.¹  Grange, Robert W.¹  Lau, Kim S.¹ 

[1] Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75235-9040, USA;Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester Medical Center, 601 Elmwood Avenue, Box 711, Rochester, NY 14642-8711, USA

关键词: Nitric oxide synthase;    cGMP;    Skeletal muscle;    Vascular smooth muscle;    NO;    nitric oxide;    NOS;    nitric oxide synthase;    nNOS;    neuronal nitric oxide synthase;    eNOS;    endothelial nitric oxide synthase;    iNOS;    inducible nitric oxide synthase;    sGC;    soluble guanylyl cyclase;    RLC;    regulatory light chain;    L-NMMA;    N G-monomethyl-l-arginine;    PSS;    physiological salt solution;    NLA;    N G-nitro-l-arginine;    [Ca2+]i;    intracellular calcium;    PVDF;    polyvinylidene fluoride;    SNP;    sodium nitroprusside;    HRP;    horse radish peroxidase;    EDTA;    ethylenediaminetetraacetic acid;    TLCK;    N-α-p-tosyl-l-lysine chloromethyl ketone;    CHAPS;    3-[(3-chlolamido propyl)dimethyl-ammonia]-1-propane sulfonate;    Hz;    hertz;    DMD;    Duchenne's muscular dystrophy;   

DOI  :  10.1016/S0014-5793(98)00728-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitric oxide generated by neuronal nitric oxide synthase in contracting skeletal muscle fibers may regulate vascular relaxation via a cGMP-mediated pathway. Neuronal nitric oxide synthase content is greatly reduced in skeletal muscles from mdx mice. cGMP formation increased in contracting extensor digitorum longus muscles in vitro from C57 control, but not mdx mice. The increase in cGMP content was abolished with N ^G-nitro-l-arginine. Sodium nitroprusside treatment increased cGMP levels in muscles from both C57 and mdx mice. Skeletal muscle contractions also inhibited phenylephrine-induced phosphorylation of smooth muscle myosin regulatory light chain. Arteriolar dilation was attenuated in contracting muscles from mdx but not C57 mice. NO generated in contracting skeletal muscle may contribute to vasodilation in response to exercise.

【 授权许可】

Unknown