Anti-Mycobacterium tuberculosis activity of fungus Phomopsis stipata

Revista de microbiologia

Sordi, Renata¹  Prince, Karina Andrade de² 

[1] Faculdades Integradas Pitágoras, Montes Claros, Brasil;Universidade Estadual Paulista, Araraquara, Brasil

关键词: Phomopsis stipata;    antimycobacterial activity;    Mycobacterium tuberculosis.;   

DOI  :  10.1590/S1517-83822012000100024

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Our purpose was to determine the anti-Mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus Phomopsis stipata (Lib.) B. Sutton, (Diaporthaceae), cultivated in different media. The antimycobacterial activity was assessed through the Resazurin Microtiter Assay (REMA) and the cytotoxicity test performed on macrophage cell line. The extracts derived from fungi grown on Corn Medium and Potato Dextrose Broth presented the smallest values of Minimum Inhibitory Concentration (MIC) and low cytotoxicity, which implies a high selectivity index. This is the first report on the chemical composition and antitubercular activity of metabolites of P. stipata, as well as the influence of culture medium on these properties.

【 授权许可】

CC BY-NC