A recombinant adenovirus expressing the E protein of duck Tembusu virus induces protective immunity in duck

The Journal of Veterinary Medical Science

Rui WANG¹  Jingyu TANG²  Dongdong YIN³ 

[1] Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei 230036, China;College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China

关键词: duck Tembusu virus;    E protein;    experimental infection;    protection;    recombinant adenovirus;   

DOI  :  10.1292/jvms.18-0036

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Duck Tembusu virus disease, caused by the duck Tembusu virus (DTMUV), can lead to a severe reduction in egg production and growth retardation in laying ducks and ducklings, respectively. In this study, we engineered a novel recombinant adenovirus expressing the E protein of DTMUV (rAd-E) in AAV-293 cells (analyzed by western blot and indirect immunofluorescence assays). Intramuscular immunization of Cherry Valley ducks with rAd-E was performed to evaluate host cellular and humoral immune responses. Compared to the phosphate-buffered saline administered group and the negative control wild-type adenovirus (wtAd) group, the rAd-E vaccinated group showed increased cellular and humoral responses. The results from the cytokine release and lymphocyte proliferation assays showed that rAd-E induced a stronger cellular immune response than the control group (P<0.01), 4 weeks after primary immunization. The results of enzyme-linked immunosorbent and virus neutralization assays showed that rAd-E induced higher titers of specific neutralizing antibodies, 2 weeks after primary immunization. The DTMUV challenge experiment showed a higher survival rate (80%) of ducks in the rAd-E group, when challenged with 0.5 ml (ELD50=10−2.67/0.2 ml) of the DTMUV strain AH-F10. These results indicate that rAd-E effectively protects ducks against DTMUV infection. Therefore, rAd-E could be a vaccine candidate to provide an effective and safe method for prevention and control of DTMUV infection.

【 授权许可】

Unknown