High rate misidentification of biochemically determined Streptococcus isolates from swine clinical specimens

The Journal of Veterinary Medical Science

Sarawan KAEWMONGKOL¹  Tanyanant KAMINSONSAKUL²  Nattakan MEEKHANON³  Siriporn KONGSOI⁴  Pichai JIRAWATTANAPONG⁵  Suksan CHUMSING⁶ 

[1] Department of Farm Resources and Production Medicines, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand;Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand;Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand;Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan;Kamphaengsaen Veterinary Diagnostic Unit, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand;Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan

关键词: diagnosis;    identification;    Streptococcus;    Streptococcus suis;    swine;   

DOI  :  10.1292/jvms.18-0678

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

In this study, 22 bacterial isolates from swine necropsy specimens, which were biochemically identified as Streptococcus suis and other Streptococcus species, were re-examined using species-specific PCR for authentic S. suis and 16S rRNA gene sequencing for the verification of the former judge. Identification of S. suis on the basis of biochemical characteristics showed high false-positive (70.6%) and false-negative (60%) rates. The authentic S. suis showed various capsular polysaccharide synthesis gene types, including type 2 that often isolated from human cases. Five of 22 isolates did not even belong to the genus Streptococcus. These results suggested that the misidentification of the causative pathogen in routine veterinary diagnosis could be a substantial obstacle for the control of emerging infectious diseases.

【 授权许可】

Unknown