Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Revista de microbiologia

Miyata, Marcelo¹ 

[1] Universidade Estadual Paulista, Araraquara, Brasil

关键词: DNA extraction;    Mycobacterium tuberculosis;    thermolysis;    CTAB.;   

DOI  :  10.1590/S1517-83822011000200045

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

【 授权许可】

CC BY-NC