DNA extraction from Ganoderma isolates of PT Socfindo collections

4th International Conference on Biological Sciences and Biotechnology

Hayati, R.^1 ; Basyuni, M.^2 ; Purba, A.^1 ; Arifianto, D.^3 ; Syahputra, I.^3 ; Chalil, D.^4 ; Bimantara, Y.^2

Graduate School of Agroteknologi, Faculty of Agriculture, Universitas Sumatera Utara, Jl. Dr. Sofyan No. 3, Medan, North-Sumatera

20155, Indonesia^1

Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1, Medan, North-Sumatera

20155, Indonesia^2

Laboratory of Biomolecular of PT Socfin Indonesia, Martebing, Dolok Masihul, Tebing Tinggi, North-Sumatra

20991, Indonesia^3

Department of Agribusiness, Faculty of Agriculture, Universitas Sumatera Utara, Medan, North-Sumatera

20155, Indonesia^4

关键词: Coconut trees;    DNA extraction;    DNA extracts;    Dna purities;    Extraction efficiencies;    Ganoderma sp;    Phenolic acids;    Quantity ratio;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/305/1/012041/pdf
DOI  :  10.1088/1755-1315/305/1/012041

来源: IOP

【 摘 要 】

Method of CTAB (cetyltrimethylethylammonium bromide) is prevalent for low-cost DNA extraction with many published variations for other plants and tissues, including fungi Ganoderma. The quantity of DNA extracts from the Ganoderma sp fungus from a coconut tree and rubber tree with concentration extraction efficiency and the quality of purity wholeness varied with different DNA characterizations. DNA purity was measured using UV spectroscopy, where a ratio of 260 nm, 280 nm 320 nm was an indicator of different contaminants. The quantity of DNA from the isolate is indicated by 31 ng/ul until 133 ng/ul and range of ratio from 1,279 until 3,6654. The DNA extraction quantity ratio value obtained is below, and above the average, for absorbance 260 nm, 280 nm and 320 nm varies between 1.5 until 1.8 which is an indicator of DNA purity. Pure DNA isolate without contaminants is only found in sample isolate Ganoderma sp 141.1 from coconut tree with a ratio value of 2.2707. Furthermore on the other samples thought to be still contaminated with phenols, salts, proteins, polysaccharide or RNA still co-extracted with phenolic acid (chloroform phenol).