Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Brazilian Journal of Microbiology

Marcelo Miyata²  Adolfo Carlos Barreto Santos²  Natália Helena Mendes¹  Eunice Atsuko Cunha¹  Fernando Augusto Fiúza De Melo¹  Clarice Queico Fujimura Leite¹ 

[1] ,Universidade Estadual Paulista Faculdade de Ciências Farmacêuticas Araraquara SP ,Brasil

关键词: DNA extraction;    Mycobacterium tuberculosis;    thermolysis;    CTAB;   

DOI  :  10.1590/S1517-83822011000200045

来源: SciELO

【 摘 要 】

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

【 授权许可】

CC BY-NC   
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