Short communication: Occurrence of angular leaf spot caused by Pseudocercospora griseola in Phaseolus vulgaris in Asturias, Spain

Spanish Journal of Agricultural Research

Elena Landeras¹  Estefanía Trapiello² 

[1] Laboratorio de Sanidad Vegetal, Consejería de Desarrollo Rural y Recursos Naturales del Principado de Asturias. C/ Lucas Rodríguez Pire 4-bajo, 33011 Oviedo, Asturias;Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA). Ctra. de Oviedo s/n,33300 Villaviciosa, Asturias

关键词: bean;    Phaeoisariopsis griseola;    ALS;    ITS;    molecular identification;   

DOI  :  10.5424/sjar/2017153-10798

学科分类：农业科学（综合）

来源: I N I A

【 摘 要 】

Angular leaf spot (ALS) symptoms were observed in 2015 in common bean fields at four locations in Asturias, NW Spain. This disease is frequent in tropical areas and we have no record of its presence in our region, at least in the last 30 years. However, since its detection its presence in the crops has been increasing. Symptoms were necrotic spots on leaves and reddish-brown to black circular spots on pods, stems, branches and petioles. The damage observed in the mentioned crops was highly variable (between 60% and 100% affected leaves), being most severe in crops where no agrochemical treatment were applied. Three strains were selected and identified based in morphological features asPseudocercospora griseola . The ITS region was amplified by PCR obtaining a sequence that was identical for the three isolates (Acc. No. LT222499). This sequence showed 99-100% similarity with those deposited in databases corresponding toP. griseola.To fulfill Koch's postulates, a pathogenicity test was carried out in two common bean cultivars (‘Andecha’ and ‘Maruxina’).P. griseolawas re-isolated from inoculated plants and not from control plants. In cv. ‘Andecha’, chlorosis was observed in all the inoculated plants, before the appearance of spots. Consequently this is the first confirmed report of this pathogen in our region.

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