Chlamydia pan-genomic analysis reveals balance between host adaptation and selective pressure to genome reduction

BMC Genomics

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[1] 0000 0000 9559 0613, grid.78028.35, Microbiology and Virology Department, Pirogov Russian National Research Medical University, Moscow, Russia;0000 0001 2192 9124, grid.4886.2, Kharkevich Institute for Information Transmission Problems, RAS, Moscow, Russia;0000 0001 2192 9124, grid.4886.2, Kharkevich Institute for Information Transmission Problems, RAS, Moscow, Russia;0000 0004 0495 846X, grid.4709.a, current address: European Molecular Biology Laboratory, Heidelberg, Germany;0000 0001 2192 9124, grid.4886.2, Kharkevich Institute for Information Transmission Problems, RAS, Moscow, Russia;0000 0004 0555 3608, grid.454320.4, Center for Life Sciences, Skolkovo Institute of Science and Technology, Moscow, Russia;0000 0001 2192 9124, grid.4886.2, Vavilov Institute of General Genetics, RAS, Moscow, Russia;0000 0001 2192 9124, grid.4886.2, Kharkevich Institute for Information Transmission Problems, RAS, Moscow, Russia;0000 0004 0555 3608, grid.454320.4, Center for Life Sciences, Skolkovo Institute of Science and Technology, Moscow, Russia;0000 0004 0578 2005, grid.410682.9, Faculty of Computer Science, Higher School of Economics, Moscow, Russia;0000 0001 2192 9124, grid.4886.2, Kharkevich Institute for Information Transmission Problems, RAS, Moscow, Russia;0000000404312247, grid.33565.36, current address: Institute of Science and Technology Austria, Klosterneuburg, Austria;0000 0001 2289 6897, grid.15447.33, Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia;0000000121581746, grid.5037.1, current address: Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, Stockholm, Sweden;0000 0004 0555 3608, grid.454320.4, Center for Life Sciences, Skolkovo Institute of Science and Technology, Moscow, Russia;

关键词: Chlamydia;    Intracellular pathogens;    Pan-genome;    Genome evolution;    Comparative genomics;    PmpG;   

DOI  :  10.1186/s12864-019-6059-5

来源: publisher

【 摘 要 】

BackgroundChlamydia are ancient intracellular pathogens with reduced, though strikingly conserved genome. Despite their parasitic lifestyle and isolated intracellular environment, these bacteria managed to avoid accumulation of deleterious mutations leading to subsequent genome degradation characteristic for many parasitic bacteria.ResultsWe report pan-genomic analysis of sixteen species from genus Chlamydia including identification and functional annotation of orthologous genes, and characterization of gene gains, losses, and rearrangements. We demonstrate the overall genome stability of these bacteria as indicated by a large fraction of common genes with conserved genomic locations. On the other hand, extreme evolvability is confined to several paralogous gene families such as polymorphic membrane proteins and phospholipase D, and likely is caused by the pressure from the host immune system.ConclusionsThis combination of a large, conserved core genome and a small, evolvable periphery likely reflect the balance between the selective pressure towards genome reduction and the need to adapt to escape from the host immunity.

【 授权许可】

CC BY