Nkx2.5 marks angioblasts that contribute to hemogenic endothelium of the endocardium and dorsal aorta

eLife

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[1] Department of Genetics and Developmental Biology, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel;Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel;Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia;Institute for Stem Cell and Regenerative Medicine, The University of Washington, Seattle, United States;Key Laboratory of Computational Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China;School of Medical Sciences, Sydney Medical School, The University of Sydney, Westmead, Australia;Embryology Unit, Children’s Medical Research Institute, Westmead, Australia;State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China;Victor Chang Cardiac Research Institute, Sydney, Australia;Victor Chang Cardiac Research Institute, Sydney, Australia;St. Vincent’s Clinical School, School of Biological and Biomolecular Sciences, University of New South Wales, Kensington, Australia;

关键词: Nkx2.5;    hemogenic endothelium;    cardiogenesis;    cardiogenesis;    hemangioblasts;    Nkx2.5;   

DOI  :  10.7554/eLife.20994

来源: publisher

【 摘 要 】

10.7554/eLife.20994.001Novel regenerative therapies may stem from deeper understanding of the mechanisms governing cardiovascular lineage diversification. Using enhancer mapping and live imaging in avian embryos, and genetic lineage tracing in mice, we investigated the spatio-temporal dynamics of cardiovascular progenitor populations. We show that expression of the cardiac transcription factor Nkx2.5 marks a mesodermal population outside of the cardiac crescent in the extraembryonic and lateral plate mesoderm, with characteristics of hemogenic angioblasts. Extra-cardiac Nkx2.5 lineage progenitors migrate into the embryo and contribute to clusters of CD41+/CD45+ and RUNX1+ cells in the endocardium, the aorta-gonad-mesonephros region of the dorsal aorta and liver. We also demonstrated that ectopic expression of Nkx2.5 in chick embryos activates the hemoangiogenic gene expression program. Taken together, we identified a hemogenic angioblast cell lineage characterized by transient Nkx2.5 expression that contributes to hemogenic endothelium and endocardium, suggesting a novel role for Nkx2.5 in hemoangiogenic lineage specification and diversification.DOI: http://dx.doi.org/10.7554/eLife.20994.001

【 授权许可】

CC BY