Downregulation of CDKL1 suppresses neuroblastoma cell proliferation, migration and invasion

Cellular & Molecular Biology Letters

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[1] 0000 0004 0632 3409, grid.410318.f, Eye Hospital, China Academy of Chinese Medical Sciences, No 33 Lugu Road, Shijingshan district, 100040, Beijing, China;Yinan Branch of Qilu Hospital of Shandong University, Linyi, Shandong, China;

关键词: Neuroblastoma;    SH-SY5Y;    CDKL1;    Proliferation;    Migration;    Invasion;   

DOI  :  10.1186/s11658-019-0139-z

来源: publisher

【 摘 要 】

BackgroundCyclin-dependent kinase-like 1 (CDKL1) is a member of the cell division control protein 2-related serine–threonine protein kinase family. It is known to occur in various malignant tumors, but its role in neuroblastoma (NB) remains unclear.MethodsWe constructed a CDKL1-silenced NB cell strain (SH-SY5Y) and used real-time PCR and western blotting to confirm the silencing. Functional analyses were performed using the MTT, colony-formation, FACS, wound-healing and transwell invasion assays.ResultsThe expression of CDKL1 was significantly upregulated in NB tissue as compared to the adjacent normal tissue. CDKL1 knockdown significantly suppressed cell viability and colony formation ability. It also induced cell cycle G0/G1 phase arrest and apoptosis, and suppressed the migration and invasion ability of SH-SY5Y cells. CDKL1 knockdown decreased the CDK4, cyclin D1 and vimentin expression levels, and increased the caspase-3, PARP and E-cadherin expression levels in SH-SY5Y cells.ConclusionsOur findings suggest that CDKL1 plays an important role in NB cell proliferation, migration and invasion. It might serve as a potential target for NB therapy.

【 授权许可】

CC BY