Tetrahydroxy stilbene glucoside alleviates palmitic acid-induced inflammation and apoptosis in cardiomyocytes by regulating miR-129-3p/Smad3 signaling

Cellular & Molecular Biology Letters

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[1] 0000 0001 0709 0000, grid.411854.d, Department of Cardiovascular Medicine, Wuhan No. 6 Hospital, Hospital Affiliated to Jianghan University, No. 168, Xianggan Road, 430016, Wuhan, People’s Republic of China;0000 0001 0709 0000, grid.411854.d, Department of Pharmacy, Wuhan No. 6 Hospital, Hospital Affiliated to Jianghan University, No. 168, Xianggan Road, 430016, Wuhan, People’s Republic of China;

关键词: Tetrahydroxy stilbene glucoside;    miR-129-3p;    Smad3;    Cardiomyocytes;    Inflammation;    Apoptosis;   

DOI  :  10.1186/s11658-018-0125-x

来源: publisher

【 摘 要 】

ObjectiveTetrahydroxy stilbene glucoside (TSG) has been reported to exert a cytoprotective effect against various toxicants. However, the function and mechanism of TSG in palmitic acid (PA)-induced inflammation and apoptosis in cardiomyocytes are still unknown. The present study was designed to investigate the post-transcriptional mechanism in TSG-treated cardiomyocytes’ inflammation and apoptosis induced by PA.MethodsThe mRNA and protein levels were assayed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting, respectively. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Cell proliferation was analyzed by CCK-8 assay. Annexin V-fluorescein isothiocyanate/polyimide (annexin V-FITC/PI) staining was used to evaluate apoptosis using flow cytometry.ResultsTSG restricted the detrimental effects, including the activated inflammatory response and apoptosis, of PA in cardiomyocytes, as well as the up-regulation of miR-129-3p and down-regulation of p-Smad3 expression. In addition, bioinformatics and experimental analysis suggested that Smad3 was a direct target of miR-129-3p, which could inhibit or enhance the expression of p-Smad by transfection with miR-129-3p mimics or inhibitors, respectively. Furthermore, our results demonstrated that overexpression of Smad3 reversed the inhibition of inflammation and apoptosis by overexpression of miR-129-3p in PA-stimulated cardiomyocytes.ConclusionTSG targeted to miR-129-3p/Smad3 signaling inhibited PA-induced inflammation and apoptosis in cardiomyocytes.

【 授权许可】

CC BY