An Approach for Improvement of Carbon Fiber Technique to Study Cardiac Cell Contractility

International Conference on Nanomaterials and Biomaterials 2017

材料科学;物理学;生物科学

Myachina, T.^1 ; Khokhlova, A.^1,2 ; Antsygin, I.^1 ; Lookin, O.^1,2

Ural Federal University, Ekaterinburg, Russia^1

Institute of Immunology and Physiology, Russian Academy of Sciences, Ekaterinburg, Russia^2

关键词: Cardiac cell;    Cardiomyocytes;    Cell lengths;    End-diastolic;    Linear coefficients;    Physiological condition;    Quality indices;    Sarcomere length;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/350/1/012011/pdf
DOI  :  10.1088/1757-899X/350/1/012011

来源: IOP

【 摘 要 】

The technologies to study cardiac cell mechanics in near-physiological conditions are limited. Carbon fiber (CF) technique is a unique tool to study single cardiomyocyte contractility. However, the CF adhesion to a cell is limited and it is difficult to control CF sliding occurred due to inappropriate adhesion. In this study, we present a CF adhesion quality index - a linear coefficient (slope) derived from "end-diastolic cell length - end-diastolic sarcomere length" relationship. Potential applicability of this index is demonstrated on isolated rat and guinea pig ventricular cardiomyocytes. Further improvement of the approach may help to increase the quality of the experimental data obtained by CF technique.