Bioimaging Tools Based on Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanoparticles: Design and Characterization of the Fluorescent Properties

Nanoscale Research Letters

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[1] 0000 0000 8868 5198, grid.183446.c, Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 31 Kashirskoye Shosse, 115409, Moscow, Russian Federation;0000 0000 8868 5198, grid.183446.c, Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 31 Kashirskoye Shosse, 115409, Moscow, Russian Federation;0000 0004 1937 0618, grid.11667.37, Laboratoire de Recherche en Nanosciences (LRN-EA4682), Université de Reims Champagne-Ardenne, 51 rue Cognacq Jay, 51100, Reims, France;V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, 1 Schukinskaya str, 123182, Moscow, Russian Federation;

关键词: Polymer microcapsules;    Polyelectrolytes;    Quantum dots;    Bioimaging;   

DOI  :  10.1186/s11671-019-2859-4

来源: publisher

【 摘 要 】

Fluorescent imaging is a widely used technique for detecting and monitoring the distribution, interaction, and transformation processes at molecular, cellular, and tissue level in modern diagnostic and other biomedical applications. Unique photophysical properties of fluorescent semiconductor nanocrystals “quantum dots” (QDs) make them advanced fluorophores for fluorescent labeling of biomolecules or optical encoding of microparticles to be used as bioimaging and theranostic agents in targeted delivery, visualization, diagnostics, and imaging. This paper reports on the results of development of an improved approach to the optical encoding of polyelectrolyte microcapsules with stable, covered with the multifunctional polyethyleneglycol derivatives water-soluble QDs, as well as characterization of the optical properties, morphological and structural properties of the encoded microcapsules. The embedding of QDs into the polymer microcapsule membrane through layer-by-layer deposition on a preliminarily formed polymeric polyelectrolyte shell makes it possible to obtain bright fluorescent particles with an adapted charge and size distribution that are distinctly discernible by flow cytometry as individual homogeneous populations. The fluorescent microcapsules developed can be used in further designing bioimaging and theranostic agents sensitive to various external stimuli along with photoexcitation.

【 授权许可】

CC BY