Cytotoxic Activity of Methoxy-4’amino Chalcone Derivatives Against Leukemia Cell Lines

MCBS (Molecular and Cellular Biomedical Sciences)

article

Arina Novilla¹  Mustofa Mustofa²  Indwiani Astuti²  Jumina Jumina³  Hery Suwito⁴ 

[1] Doctoral Program, Faculty of Medicine, Gadjah Mada University, Yogyakarta;Faculty of Medicine, Gadjah Mada University, Yogyakarta;Faculty of Chemistry, Gadjah Mada University, Yogyakarta;Doctoral Program, Faculty of Chemistry, Gadjah Mada University, Yogyakarta

关键词: anticancer;    chalcone derivatives;    methoxy-4’-amino chalcone;    leukemia;    cytotoxic;    selectivity;   

DOI  :  10.21705/mcbs.v3i1.44

学科分类：内科医学

来源: Cell and BioPharmaceutical Institute

【 摘 要 】

Background: Chemotherapy is a common treatment for leukemia as well as in other cancer treatment. The lack of tumor selectivity and development of multi-drug resistance by chemotherapy caused the development of new strategy in cancer treatment become a pressing need. This study was performed to evaluate the anticancer activity and selectivity of seven derivatives of chalcones against K562 and HL-60 leukemia cell lines. Materials and Methods: The cytotoxicity of chalcone’s seven derivatives (compound 1-7) was tested by using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method. The percentage of cell mortality data was calculated then the IC₅₀ was analyzed using probit analysis (SPSS 17). The selectivity index (SI) then calculated from IC₅₀ ratio of normal lymphocyte cells and cancerous cells line (HL-60 and K562). Results: The IC₅₀ of almost all seven tested compounds were lower in HL-60 cell lines than K562 cell lines, except for Compound 7. The number and position of methoxy groups in chalcone derivatives influenced the anticancer and cancer selectivity of chalcone derivatives. Conclusion: The results revealed that the number and position of methoxy groups in chalcone derivatives influenced the anticancer and cancer selectivity of chalcone derivatives.

【 授权许可】

CC BY-NC