Virus‐specific CD4 and CD8 T cell responses in the absence of Th1‐associated transcription factors

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: cytokines;    memory;    effector;    Tbet;    STAT4;    LCMV;   

DOI  :  10.1189/jlb.0813429

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

EffectorandmemoryCD4andCD8Tcellresponsesarecriticalforthecontrolofmanyintracellularpathogens.Thedevelopmentofthesepopulationsisgovernedbytranscriptionfactorsthatmolecularlycontroltheirdifferentiation,function,andmaintenance.TwotranscriptionfactorsknowntobeinvolvedintheseprocessesareTbetandSTAT4.AlthoughTbethasbeenshowntoregulateCD8TcellfatedecisionsandeffectorCD4Tcellchoices,thecontributionofSTAT4islesswellunderstood.Toaddressthis,weexaminedtheimpactofSTAT4onTcellresponsesinthepresenceorabsenceofTbet,followingLCMVinfectionbyusingmicelackingTbet,STAT4,orbothtranscriptionfactors.STAT4wasnotrequiredforTbetorEomesexpression;however,virus‐specificeffectorCD8Tcellsareskewedtowardamemory‐precursorphenotypeintheabsenceofSTAT4.ThisalteredproportionofmemoryprecursorsdidnotresultinanincreaseinmemoryCD8Tcellsaftertheresolutionoftheinfection.Wealsodemonstratethatvirus‐specificeffectorandmemoryCD4TcellsformedindependentlyofTbetandSTAT4,althoughaslightreductioninthenumberofantigen‐specificCD4Tcellswasapparentinmicelackingbothtranscriptionfactors.Collectively,wehavediscovereddistinctrolesforTbetandSTAT4inshapingthephenotypeandfunctionofvirus‐specificTcellresponses...

【 授权许可】

CC BY