Memory deficit associated with increased brain proinflammatory cytokine levels and neurodegeneration in acute ischemic stroke

Arquivos de Neuro-Psiquiatria

Bruno Silva¹  Larissa Sousa¹  Aline Miranda¹  Anilton Vasconcelos¹  Helton Reis¹  Lucíola Barcelos¹  Rosa Arantes¹  Antonio Teixeira¹  Milene Alvarenga Rachid¹ 

关键词: brain;    ischemia;    memory;    inflammation;    cytokines;    encéfalo;    isquemia;    memória;    inflamação;    citocinas;   

DOI  :  10.1590/0004-282X20150083

来源: SciELO

【 摘 要 】

The present study aimed to investigate behavioral changes and neuroinflammatory process following left unilateral common carotid artery occlusion (UCCAO), a model of cerebral ischemia. Post-ischemic behavioral changes following 15 min UCCAO were recorded 24 hours after reperfusion. The novel object recognition task was used to assess learning and memory. After behavioral test, brains from sham and ischemic mice were removed and processed to evaluate central nervous system pathology by TTC and H&E techniques as well as inflammatory mediators by ELISA. UCCAO promoted long-term memory impairment after reperfusion. Infarct areas were observed in the cerebrum by TTC stain. Moreover, the histopathological analysis revealed cerebral necrotic cavities surrounded by ischemic neurons and hippocampal neurodegeneration. In parallel with memory dysfunction, brain levels of TNF-a, IL-1b and CXCL1 were increased post ischemia compared with sham-operated group. These findings suggest an involvement of central nervous system inflammatory mediators and brain damage in cognitive impairment following unilateral acute ischemia.

【 授权许可】

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