LL‐37 modulates human neutrophil responses to influenza A virus

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: innate immunity;    antimicrobial peptides;    FPRL‐;    1;    defensin;   

DOI  :  10.1189/jlb.4A1113-604RR

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Recentstudieshaveshownthatthehumancathelicidin,LL‐37,hasantiviralactivityagainstIAVinvitroandinvivo.NeutrophilsareimportantcellularcomponentsoftheinitialinnateresponsetoIAVinfection.Inadditiontoitsdirectantimicrobialactivities,LL‐37hasimportantimmunomodulatoryeffects.Inthisstudy,weexplorehowLL‐37affectsinteractionsofIAVwithhumanneutrophils.LL‐37didnotalterneutrophiluptakeofIAVbutsignificantlyincreasedneutrophilH2O2responsestothevirus.IAVstimulatedproductionofNETsinvitro,andthisresponsewasincreasedbypreincubatingtheviruswithLL‐37.NADPH‐oxidaseblockadedidnotreduceIAV‐inducedNETformationortheincreasedNETresponsestimulatedbyLL‐37+IAV.TheincreasedrespiratoryburstandNETresponseswere,however,inhibitedbypreincubatingcellswithaformylpeptidereceptorblocker,indicatingthatLL‐37engagesthesereceptorswhencomplexedwithIAV.ResponsestoIAValonewerenotinhibitedbyformylpeptidereceptorblockade.IthasbeenreportedthatLL‐37reducesproinflammatorycytokineresponsesduringIAVinfectioninvivo.WenowshowthatIAValonepotentiatedreleaseofIL‐8fromneutrophils,andpreincubationwithLL‐37reducedIAV‐stimulatedIL‐8release.TheseresultsconfirmthatLL‐37modulateshumanneutrophilresponsestoIAVinadistinctivemannerandcouldhaveimportantbearingontheprotectiveeffectsofLL‐37duringIAVinfectioninvivo...

【 授权许可】

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