IL-6 Production Stimulated by CD14+ Monocytes-paracrined IL-1β does not Contribute to the Immunosuppressive Activity of Human Umbilical Cord Mesenchymal Stem Cells

Cellular Physiology and Biochemistry

关键词: Umbilical cord mesenchymal stem cells (UC-MSCs);    IL-6;    PGE2;    IL-1β;   

DOI  :  10.1159/000338509

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background/Aims Human umbilical cord mesenchymal stem cells (hUC-MSCs) possess immunosuppressive activities but the mechanisms of such activities are not fully understood. Here, we investigated the role of IL-6, one of the characteristic factors of MSCs, in the immunoregulating effect of hUC-MSCs on CD4⁺ T lymphocytes. Methods The condition media from human peripheral blood mononuclear cells (hPBMCs) or CD14+/- cell were tested if stimulating IL-6 production by hUC-MSCs. The related signaling pathway of IL-6, and the immunosuppressive activity of IL-6 on CD4⁺ T lymphocytes were studied. Result IL-6 production was dramatically increased by hUC-MSCs when co-culturing with resting or activated hPBMCs. CD14⁺ monocytes-paracrined IL-1β promoted the secretion of IL-6 by hUC-MSCs via JNK and NF-ĸB signaling pathway. Blocking of PGE2 synthesis did not affect the secretion of IL-6, anti-IL-6 antibody was not able to reverse hUC-MSCs-mediated inhibition on CD4⁺ T lymphocytes. IL-6 did not mediate the suppressive activity of IL-1β-hUC-MSCs- PGE2 on CD4⁺ T cell. Conclusion CD14⁺ monocytes-paracrined IL-1β promotes IL-6 secretion by hUC-MSCs through activating JNK and NF-ĸB signaling pathway. However, increased IL-6 production does not contribute to immunosuppressive activity of IL-1β-hUC-MSCs- PGE2 on CD4⁺ T cells.

【 授权许可】

CC BY-NC-ND