Human monocyte derived dendritic cells express functional P2X and P2Y receptors as well as ecto‐nucleotidases

FEBS Letters

Bogdan, Cornelia²  Steinkasserer, Alexander²  Ogilvie, Adaling¹  Berchtold, Susanne²  Schuler, Gerold²  Mühl-Zürbes, Petra²  Ogilvie, Alexandra L.J.² 

[1] Department of Biochemistry, University of Erlangen, Fahrstr. 17, D-91054 Erlangen, Germany;Department of Dermatology, University of Erlangen, Hartmannstr. 14, D-91052 Erlangen, Germany

关键词: Dendritic cell;    ATP;    P2 receptor;    Ecto-nucleotidase;    DC;    dendritic cell;    EGTA;    ethylene glycol-O;    O′-bis(2-aminoethyl)-N;    N;    N′;    N′-tetraacetic acid);    FITC;    fluorescein isothiocyanate;    GM-CSF;    granulocyte-macrophage colony stimulating factor;    IL-1β;    interleukin 1β;    IL-6;    interleukin 6;    IL-4;    interleukin 4;    PGE2;    prostaglandin E2;    TNF-α;    tumor necrosis factor α;   

DOI  :  10.1016/S0014-5793(99)01197-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the expression and function of P2 receptors and ecto-nucleotidases on human monocyte derived dendritic cells (DC). In addition we analyzed the effect of extracellular ATP on the maturation of DC. By RT-PCR, DC were found to express mRNA for several P2X (P2X1, P2X4, P2X5, P2X7) and P2Y (P2Y1, P2Y2, P2Y4, P2Y5, P2Y6, P2Y10, P2Y11) receptors. As shown by FURA-2 measurement, triggering of P2 receptors resulted in an increase in free intracellular Ca²⁺. In combination with Tumor necrosis factor-α, ATP increased the expression of the DC surface markers CD80, CD83 and CD86 indicating a maturation promoting effect. DC expressed the ecto-apyrase CD39 and the ecto-5′-nucleotidase CD73 as demonstrated by RT-PCR. Extracellular ATP was rapidly hydrolyzed by these ecto-enzymes as shown by separation of ³H-labeled ATP metabolites using a thin layer technique. These data suggest that ATP acts as a costimulatory factor on DC maturation.

【 授权许可】

Unknown