Alteration of the Transcriptional Profile of Human Embryonic Kidney Cells by Transient Overexpression of Mouse TRPM7 Channels

Cellular Physiology and Biochemistry

关键词: TRPM7;    Microarray;    Transcriptome;    Differential Expression;    Cellular Pathway;   

DOI  :  10.1159/000327958

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background TRPM7 is a cation channel containing a functional kinase domain. The functional activity of TRPM7 is essential for cell viability and growth, and its expression is up-regulated in certain pathological conditions, such as ischemia. Methods In order to assess the effects of TRPM7 activity on cellular gene expression, inducible HEK293 cell-lines harboring the wild-type mouse TRPM7 and a mutant lacking the kinase domain were established. The wild-type and the non-functional TRPM7 channels were induced transiently and the comparative changes in cellular transcription were investigated. Results From the genome-scale analysis using a human genome expression microarray, we identified 951 genes altered in transcription significantly and specifically by the expression of the functional TRPM7 channel. Conclusion By analyzing the genes differentially expressed by TRPM7, we were able to provide potential target proteins and to delineate the cellular pathways affected by the channel function.

【 授权许可】

CC BY-NC-ND