A proteomic analysis of serum-derived exosomes in rheumatoid arthritis

BMC Rheumatology

Naoya Suematsu¹  Kazuki Okamoto¹  Toshiyuki Sato¹  Masaaki Sato¹  Hirotaka Tsuno¹  Atsushi Hashimoto¹  Toshihiro Matsui²  Mitsumi Arito²  Kazuki Omoteyama²  Tomohiro Kato³  Manae S. Kurokawa⁴  Shigeto Tohma⁵ 

[1] Clinical Proteomics and Molecular Medicine, St. Marianna University Graduate School of Medicine, Kawasaki, Japan;Department of Rheumatology, National Hospital Organization Sagamihara National Hospital, Sagamihara, Japan;Disease Biomarker Analysis and Molecular Regulation, St. Marianna University Graduate School of Medicine, Kawasaki, Japan;National Hospital Organization Tokyo National Hospital, Tokyo, Japan;The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan

关键词: Exosome;    Proteomics;    Rheumatoid arthritis;    Osteoarthritis;    Toll like receptor 3;   

DOI  :  10.1186/s41927-018-0041-8

学科分类：内科医学

来源: BioMed Central

【 摘 要 】

To understand the roles of serum exosomes in rheumatoid arthritis (RA), we comprehensively investigated the protein profiles of serum exosomes in patients with RA. Exosomes were isolated from serum samples obtained from 33 patients (12 with active RA [aRA], 11 with inactive RA [iRA], 10 with osteoarthritis [OA]) and 10 healthy donors (HLs). Proteins extracted from the exosomes were separated by two-dimensional differential gel electrophoresis (2D-DIGE) and identified by mass spectrometry. In total, 204 protein spots were detected by 2D-DIGE. In the aRA, iRA, and OA groups, 24, 5, and 7 spots showed approximately ≥ ±1.3-fold intensity differences compared with the HL group, respectively. We were able to identify proteins in six protein spots. Among them, the protein spot identified as Toll-like receptor 3 (TLR3) showed approximately 6-fold higher intensity in the aRA group than in the other groups. Patients with active RA possessed considerably different protein profiles of serum exosomes from patients with iRA, patients with OA, and healthy donors. The unique protein profile of serum exosomes, such as the possession of abundant TLR3 fragments, may reflect the pathophysiology of active RA.

【 授权许可】

CC BY