CXCL4-induced plaque macrophages can be specifically identified by co-expression of MMP7+S100A8+in vitro and in vivo:

Innate Immunity

ChristianErbel¹ 

关键词: Atherosclerosis;    cell differentiation;    chemokines;    inflammation;    macrophages;   

DOI  :  10.1177/1753425914526461

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Macrophage heterogeneity in human atherosclerotic plaques has been recognized; however, markers for unequivocal identification of some subtypes are lacking. We found that the platelet chemokine CXCL4 induces a unique macrophage phenotype, which we proposed to call ‘M4’. Here, we sought to identify suitable markers that identify M4 macrophages in vitro and in vivo. Using a stringent algorithm, we identified a set of potential markers from transcriptomic data derived from polarized macrophages. We specifically focused on matrix metalloproteinase (MMP)7 and S100A8, the co-expression of which has not been described in any macrophage type thus far. We found dose- and time-dependent MMP7 and S100A8 expression in M4 macrophages at the gene and protein levels. CXCL4-induced up-regulation of both MMP7 and S100A8 was curbed in the presence of heparin, which binds to CXCL4 and glycosaminoglycans, most likely representing the macrophage receptor for CXCL4. Immunofluorescence of post-mortem atherosclerotic coronary ar...

【 授权许可】

CC BY