Innate Immunity | |
CXCL4-induced plaque macrophages can be specifically identified by co-expression of MMP7+S100A8+in vitro and in vivo: | |
ChristianErbel1  | |
关键词: Atherosclerosis; cell differentiation; chemokines; inflammation; macrophages; | |
DOI : 10.1177/1753425914526461 | |
学科分类:生物科学(综合) | |
来源: Sage Journals | |
【 摘 要 】
Macrophage heterogeneity in human atherosclerotic plaques has been recognized; however, markers for unequivocal identification of some subtypes are lacking. We found that the platelet chemokine CXCL4 induces a unique macrophage phenotype, which we proposed to call ‘M4’. Here, we sought to identify suitable markers that identify M4 macrophages in vitro and in vivo. Using a stringent algorithm, we identified a set of potential markers from transcriptomic data derived from polarized macrophages. We specifically focused on matrix metalloproteinase (MMP)7 and S100A8, the co-expression of which has not been described in any macrophage type thus far. We found dose- and time-dependent MMP7 and S100A8 expression in M4 macrophages at the gene and protein levels. CXCL4-induced up-regulation of both MMP7 and S100A8 was curbed in the presence of heparin, which binds to CXCL4 and glycosaminoglycans, most likely representing the macrophage receptor for CXCL4. Immunofluorescence of post-mortem atherosclerotic coronary ar...
【 授权许可】
CC BY
【 预 览 】
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RO201901223660527ZK.pdf | 1146KB | download |