Coptis chinensis inhibits melanogenesis increasing miR-340-mediated suppression of microphathalmia-associated transcription factor

Biomedical Dermatology

Hwa Jun Cha¹  Jung-Eun Ku²  Hyun Kyung Lee⁴  Dahye Joo⁴  Seonghee Jeong⁴  Seong Jin Choi⁴  In-Sook An⁴  Shang Hun Shin⁴  Karam Kim⁴  Kyung-Yun Kim⁵  Sun-Hee Jeong⁶ 

[1] Department of Beauty Art, Faculty of Art, Suwon Women’Department of Cosmetology, Kyung-In Women’Department of Skin Care and Beauty, Osan University, Osan-si, Republic of Korea;Korea Institute of Dermatological Sciences, Cheongju-si, Republic of Korea;URG Inc., Seochogu, Republic of Korea;s University, Incheon, Republic of Korea;s University, Suwon-si, Republic of Korea

关键词: Coptis chinensis;    Tyrosinase;    Melanogenesis;    Melanin contents;    MITF;    miR-340;    Skin-whitening effect;   

DOI  :  10.1186/s41702-017-0006-3

学科分类：皮肤病学

来源: Springer

【 摘 要 】

Coptis chinensis (C. chinensis) contains various antioxidants, including berberine, epiberberlin, ferulic acid, magnoflorine, palmatine, and worenine, which have antibacterial, anti-inflammatory, haemostatic, hypotensive, and anticancer effects. In the present study, the melanogenesis-inhibiting effects of C. chinensis were investigated and the molecular mechanisms were elucidated. The melanogenesis-inhibiting effect of C. chinensis was verified by measuring melanin contents, melanogenesis-related tyrosinase activities, and mRNA and protein expression levels of tyrosinase and microphthalmia-associated transcription factor (MITF). In addition, changes in miR-340 expression by C. chinensis were verified, and the activity of the miR-340 binding site of the target MITF gene was determined using luciferase reporter assays. Assays of melanin contents showed that C. chinensis had a skin-whitening effect and controlled mRNA and protein expression levels of tyrosinase. However, C. chinensis controlled protein levels of MITF without affecting mRNA levels. Determinations of miR-340 expression, which directly influences MITF translation, showed increased miR-340 mRNA levels in the presence of C. chinensis. Finally, luciferase reporter assays of the binding site on MITF showed that C. chinensis inhibits melanogenesis by directly controlling the miR-340–MITF axis. The results of the present study verified the skin-whitening effect of C. chinensis and its molecular mechanisms and indicated that C. chinensis has high potential as an ingredient in skin-whitening cosmetics.

【 授权许可】

CC BY