Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract

BMC Complementary and Alternative Medicine

Research Article

Tsong-Min Chang¹  Wan-Yu Hsieh¹  Huey-Chun Huang²  Yu-Lin Niu³ 

[1] Department of Applied Cosmetology & Master Program of Cosmetic Science, Hung Kuang University, No. 34, Chung-Chie Rd., 43302, Shalu, Taichung City, Taiwan;Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91 Hsueh-Shih Road, 40402, Taichung, Taiwan;Niuer International Skincare Science Research Institute, 7F, No. 618, Ruiguang Rd., Neihu Dist., Taipei, Taiwan;

关键词: Magnolia grandiflora;    Melanogenesis;    Tyrosinase;    Melanin;    Antioxidant;   

DOI  :  10.1186/1472-6882-12-72

 received in 2012-02-24, accepted in 2012-06-06,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMagnolia grandiflora L. flower is wildly used in Asian as a traditional herbal medication. The purpose of the study was to investigate the antimelanogenic and antioxidant properties of Magnolia grandiflora L. flower extract. In the study, the inhibitory effects of M. grandiflora L. flower extract on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were determined spectrophotometrically. Meanwhile, the antioxidative capacity of the flower extract was also investigated.ResultsOur results revealed that M. grandiflora L. flower extract inhibit mushroom tyrosinase activity (IC50 =11.1%; v/v), the flower extract also effectively suppressed intracellular tyrosinase activity (IC50 = 13.6%; v/v) and decreased the amount of melanin (IC50 = 25.6%; v/v) in a dose-dependent manner in B16F10 cells. Protein expression level of tyrosinase and tyrosinase-related protein 1 (TRP-1) were also decreased by the flower extract. Additionally, antioxidant capacities such as ABTS+ free radical scavenging activity, reducing capacity and total phenolic content of the flower extract were increased in a dose-dependent pattern.ConclusionsOur results concluded that M. grandiflora L. flower extract decreased the expression of tyrosinase and TRP-1, and then inhibited melanogenesis in B16F10 cells. The flower extract also show antioxidant capacities and depleted cellular reactive oxygen species (ROS). Hence, M. grandiflora L. flower extract could be applied as a type of dermatological whitening agent in skin care products.

【 授权许可】

Unknown   
© Huang et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.