Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract

BMC Complementary and Alternative Medicine

Tsong-Min Chang³  Yu-Lin Niu¹  Wan-Yu Hsieh³  Huey-Chun Huang² 

[1] Niuer International Skincare Science Research Institute, 7F, No. 618, Ruiguang Rd., Neihu Dist., Taipei, Taiwan;Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91 Hsueh-Shih Road, Taichung, Taiwan, 40402;Department of Applied Cosmetology & Master Program of Cosmetic Science, Hung Kuang University, No. 34, Chung-Chie Rd., Shalu, Taichung City, Taiwan, 43302

关键词: Antioxidant;    Melanin;    Tyrosinase;    Melanogenesis;    Magnolia grandiflora L;   

Others  :  1232143
DOI  :  10.1186/1472-6882-12-72

 received in 2012-02-24, accepted in 2012-06-06,  发布年份 2012

【 摘 要 】

Background

Magnolia grandiflora L. flower is wildly used in Asian as a traditional herbal medication. The purpose of the study was to investigate the antimelanogenic and antioxidant properties of Magnolia grandiflora L. flower extract. In the study, the inhibitory effects of M. grandiflora L. flower extract on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were determined spectrophotometrically. Meanwhile, the antioxidative capacity of the flower extract was also investigated.

Results

Our results revealed that M. grandiflora L. flower extract inhibit mushroom tyrosinase activity (IC₅₀ =11.1%; v/v), the flower extract also effectively suppressed intracellular tyrosinase activity (IC₅₀ = 13.6%; v/v) and decreased the amount of melanin (IC₅₀ = 25.6%; v/v) in a dose-dependent manner in B16F10 cells. Protein expression level of tyrosinase and tyrosinase-related protein 1 (TRP-1) were also decreased by the flower extract. Additionally, antioxidant capacities such as ABTS⁺ free radical scavenging activity, reducing capacity and total phenolic content of the flower extract were increased in a dose-dependent pattern.

Conclusions

Our results concluded that M. grandiflora L. flower extract decreased the expression of tyrosinase and TRP-1, and then inhibited melanogenesis in B16F10 cells. The flower extract also show antioxidant capacities and depleted cellular reactive oxygen species (ROS). Hence, M. grandiflora L. flower extract could be applied as a type of dermatological whitening agent in skin care products.

【 授权许可】

   
2012 Huang et al.; licensee BioMed Central Ltd.

【 图 表 】

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