期刊论文详细信息
Innate Immunity
Micronutrient modulation of NF-κB in oral keratinocytes exposed to periodontal bacteria:
Michael RMilward1 
关键词: α-Lipoic acid;    epithelium;    Fusobacterium nucleatum;    periodontitis;    Porphyromonas gingivalis;   
DOI  :  10.1177/1753425912454761
学科分类:生物科学(综合)
来源: Sage Journals
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【 摘 要 】

Chronic periodontal diseases are characterised by a dysregulated and exaggerated inflammatory/immune response to plaque bacteria. We have demonstrated previously that oral keratinocytes up-regulate key molecular markers of inflammation, including NF-κB and cytokine signalling, when exposed to the periodontal bacteria Porphyromonas gingivalis and Fusobacterium nucleatum in vitro. The purpose of the current study was to investigate whether α-lipoic acid was able to abrogate bacterially-induced pro-inflammatory changes in the H400 oral epithelial cell line. Initial studies indicated that α-lipoic acid supplementation (1–4 mM) significantly reduced cell attachment; lower concentrations (<0.5 mM) enabled >85% cell adhesion at 24 h. While a pro-inflammatory response, demonstrable by NF-κB translocation, gene expression and protein production was evident in H400 cells following exposure to P. gingivalis and F. nucleatum, pre-incubation of cells with 0.5 mM α-lipoic acid modulated this response. α-Lipoic acid pre...

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