Micronutrient modulation of NF-κB in oral keratinocytes exposed to periodontal bacteria:

Innate Immunity

Michael RMilward¹ 

关键词: α-Lipoic acid;    epithelium;    Fusobacterium nucleatum;    periodontitis;    Porphyromonas gingivalis;   

DOI  :  10.1177/1753425912454761

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Chronic periodontal diseases are characterised by a dysregulated and exaggerated inflammatory/immune response to plaque bacteria. We have demonstrated previously that oral keratinocytes up-regulate key molecular markers of inflammation, including NF-κB and cytokine signalling, when exposed to the periodontal bacteria Porphyromonas gingivalis and Fusobacterium nucleatum in vitro. The purpose of the current study was to investigate whether α-lipoic acid was able to abrogate bacterially-induced pro-inflammatory changes in the H400 oral epithelial cell line. Initial studies indicated that α-lipoic acid supplementation (1–4 mM) significantly reduced cell attachment; lower concentrations (<0.5 mM) enabled >85% cell adhesion at 24 h. While a pro-inflammatory response, demonstrable by NF-κB translocation, gene expression and protein production was evident in H400 cells following exposure to P. gingivalis and F. nucleatum, pre-incubation of cells with 0.5 mM α-lipoic acid modulated this response. α-Lipoic acid pre...

【 授权许可】

CC BY