Innate Immunity | |
Involvement of NLRP10 in IL-1α induction of oral epithelial cells by periodontal pathogens: | |
Seok-JooLee1  | |
关键词: NLRP10; IL-1α; periodontitis; Tannerella forsythia; Fusobacterium nucleatum; | |
DOI : 10.1177/1753425917722610 | |
学科分类:生物科学(综合) | |
来源: Sage Journals | |
【 摘 要 】
This study investigated the pathogenesis of periodontitis and the role of nucleotide-binding oligomerization domain-like receptor protein 10 (NLRP10). The human oral epithelial cell line HOK-16B was infected with two periodontal pathogens, Tannerella forsythia and Fusobacterium nucleatum, at various MOIs. RT-PCR and immunoblotting demonstrated that infection increased mRNA and protein expression of NLRP10, respectively. The siRNA-mediated NLRP10 knockdown significantly reduced IL-1α expression and secretion. Both bacteria induced phosphorylation of ERK, JNK and p38 MAP kinases in HOK-16B cells. NLRP10 knockdown impaired ERK phosphorylation only. ERK inhibition significantly decreased the expression of T. forsythia- and F. nucleatum-induced IL-1α. Our data suggest that NLRP10 is involved in activating the ERK signalling pathway in HOK-16B cells infected with T. forsythia and F. nucleatum. This pathway likely augments the pro-inflammatory cytokine IL-1α levels, which may play a critical role in periodontitis.
【 授权许可】
CC BY
【 预 览 】
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RO201904021404167ZK.pdf | 731KB | download |