Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults

PLoS One

Christine A. F. von Arnim¹  Michael Przybylski²  Iris-Tatjana Kolassa³  Vera Maria Leirer³  Thomas Elbert³  Madalina Maftei⁴  Marilena Manea⁴  Franka Thurm⁵ 

[1] Clinical and Biological Psychology, Institute of Psychology and Education, University of Ulm, Ulm, Germany;Department of Neurology, University of Ulm, Ulm, Germany;Department of Psychology, University of Konstanz, Konstanz, Germany;Laboratory of Analytical Chemistry and Biopolymer Structure Analysis, Department of Chemistry, University of Konstanz, Konstanz, Germany;Steinbeis Research Center for Biopolymer Analysis, University of Konstanz, Konstanz, Germany

关键词: Enzyme-linked immunoassays;    Immune complex;    Alzheimer's disease;    Antibodies;    Cognitive impairment;    Matrix-assisted laser desorption ionization mass spectrometry;    Semantics;    Vision;   

DOI  :  10.1371/journal.pone.0044516

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Physiological β-amyloid autoantibodies (Aβ-autoantibodies) are currently investigated as potential diagnostic and therapeutic tools for Alzheimer’s disease (AD). In previous studies, their determination in serum and cerebrospinal fluid (CSF) using indirect ELISA has provided controversial results, which may be due to the presence of preformed Aβ antigen-antibody immune complexes. Based on the epitope specificity of the Aβ-autoantibodies, recently elucidated in our laboratory, we developed (a) a sandwich ELISA for the determination of circulating Aβ-IgG immune complexes and (b) an indirect ELISA for the determination of free Aβ-autoantibodies. This methodology was applied to the analysis of serum samples from healthy individuals within the age range of 18 to 89 years. Neuropsychological examination of the participants in this study indicated non-pathological, age-related cognitive decline, revealed especially by tests of visual memory and executive function, as well as speed-related tasks. The ELISA serum determinations showed significantly higher levels of Aβ-IgG immune complexes compared to free Aβ-autoantibodies, while no correlation with age or cognitive performance of the participants was found.

【 授权许可】

CC BY