Regulatory role of periodontal ligament fibroblasts for innate immune cell function and differentiation:

Innate Immunity

AnnaKonermann¹ 

关键词: Dendritic cells;    immunomodulation;    macrophages;    stromal cells;    periodontal ligament fibroblasts;   

DOI  :  10.1177/1753425912440598

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Innate immunity is crucial for an effective host defense against pathogenic microorganisms in periodontal tissues. As periodontal ligament (PDL) cells synthesize immunomodulatory cytokines, the aim of this in vitro study was to investigate whether these cells can interact with innate immune cells. Resting and inflammatory primed (IL-1β, TNF-α, HMGB1) human PDL cells were co-cultured with human monocyte-derived dendritic cells or macrophages. Migration, phenotypic maturation and modulation of phagocytosis of Porphyromonas gingivalis by immune cells were investigated upon co-culture with PDL cells and/or their released soluble factors. PDL cells interacted with immune cells under both non-inflammatory and inflammatory conditions. Immune cell migration was significantly enhanced by co-culture with PDL cells, which also affected their phenotypic maturation both through cell-cell contact and through released soluble mediators. The dendritic cell maturation markers CD83 and CD86 were upregulated as much as both...

【 授权许可】

CC BY