期刊论文详细信息
PLoS One
A Salmon Protein Hydrolysate Exerts Lipid-Independent Anti-Atherosclerotic Activity in ApoE-Deficient Mice
Jon Skorve1  Bodil Bjørndal1  Rolf K. Berge1  Rita Vik1  Giulia Chiesa2  Jan E. Nordrehaug2  Giulia S. Ganzetti3  Cesare R. Sirtori3  Cinzia Parolini3  Marco Busnelli3  Stefano Manzini3  Federica Dellera3  Trond Brattelid4  Sverre Holm5  Pål Aukrust5  Bente Halvorsen5 
[1] Department of Clinical Science, University of Bergen, Bergen, Norway;Department of Heart Disease, Haukeland University Hospital, Bergen, Norway;Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy;National Institute of Nutrition and Seafood Research, NIFES, Bergen, Norway;Research Institute of Internal Medicine, Rikshospitalet University Hospital, Oslo, Norway
关键词: Diet;    Lipids;    Fatty acids;    Atherosclerosis;    Blood plasma;    Inflammation;    Cholesterol;    Macrophages;   
DOI  :  10.1371/journal.pone.0097598
学科分类:医学(综合)
来源: Public Library of Science
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【 摘 要 】

Fish consumption is considered health beneficial as it decreases cardiovascular disease (CVD)-risk through effects on plasma lipids and inflammation. We investigated a salmon protein hydrolysate (SPH) that is hypothesized to influence lipid metabolism and to have anti-atherosclerotic and anti-inflammatory properties. 24 female apolipoprotein (apo) E−/− mice were divided into two groups and fed a high-fat diet with or without 5% (w/w) SPH for 12 weeks. The atherosclerotic plaque area in aortic sinus and arch, plasma lipid profile, fatty acid composition, hepatic enzyme activities and gene expression were determined. A significantly reduced atherosclerotic plaque area in the aortic arch and aortic sinus was found in the 12 apoE−/− mice fed 5% SPH for 12 weeks compared to the 12 casein-fed control mice. Immunohistochemical characterization of atherosclerotic lesions in aortic sinus displayed no differences in plaque composition between mice fed SPH compared to controls. However, reduced mRNA level of Icam1 in the aortic arch was found. The plasma content of arachidonic acid (C20∶4n-6) and oleic acid (C18∶1n-9) were increased and decreased, respectively. SPH-feeding decreased the plasma concentration of IL-1β, IL-6, TNF-α and GM-CSF, whereas plasma cholesterol and triacylglycerols (TAG) were unchanged, accompanied by unchanged mitochondrial fatty acid oxidation and acyl-CoA:cholesterol acyltransferase (ACAT)-activity. These data show that a 5% (w/w) SPH diet reduces atherosclerosis in apoE−/− mice and attenuate risk factors related to atherosclerotic disorders by acting both at vascular and systemic levels, and not directly related to changes in plasma lipids or fatty acids.

【 授权许可】

CC BY   

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