Complete Genome Sequence of the Multiresistant Taxonomic Outlier Pseudomonas aeruginosa PA7

PLoS One

Liam Elbourne¹  Sasha G. Tetu¹  Ian T. Paulsen¹  Simon Tremblay²  Paul H. Roy²  André Larouche²  Qinghu Ren³  Yasmin Mahamoud³  Robert Dodson³  Kisha Watkins³  Derek Harkins³  Ryan Shay³ 

[1] Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, New South Wales, Australia;Département de biochimie et de microbiologie, and Centre de recherche en infectiologie, Université Laval, Québec, Québec, Canada;J. Craig Venter Institute, Rockville, Maryland, United States of America

关键词: Pseudomonas aeruginosa;    Comparative genomics;    Islands;    Antibiotic resistance;    Bacteriophages;    Genomic libraries;    Carbohydrate metabolism;    Virulence factors;   

DOI  :  10.1371/journal.pone.0008842

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Pseudomonas aeruginosa PA7 is a non-respiratory human isolate from Argentina that is multiresistant to antibiotics. We first sequenced gyrA, gyrB, parC, parE, ampC, ampR, and several housekeeping genes and found that PA7 is a taxonomic outlier. We report here the complete sequence of the 6,588,339 bp genome, which has only about 95% overall identity to other strains. PA7 has multiple novel genomic islands and a total of 51 occupied regions of genomic plasticity. These islands include antibiotic resistance genes, parts of transposons, prophages, and a pKLC102-related island. Several PA7 genes not present in PAO1 or PA14 are putative orthologues of other Pseudomonas spp. and Ralstonia spp. genes. PA7 appears to be closely related to the known taxonomic outlier DSM1128 (ATCC9027). PA7 lacks several virulence factors, notably the entire TTSS region corresponding to PA1690-PA1725 of PAO1. It has neither exoS nor exoU and lacks toxA, exoT, and exoY. PA7 is serotype O12 and pyoverdin type II. Preliminary proteomic studies indicate numerous differences with PAO1, some of which are probably a consequence of a frameshift mutation in the mvfR quorum sensing regulatory gene.

【 授权许可】

CC BY