Effects of vaspin on pancreatic β cell secretion via PI3K/Akt and NF-κB signaling pathways

PLoS One

Xin Li¹  Ruixue Duan¹  Shiwei Liu²  Jiaxin Zhang³  Qi Zhang³  Fang Du³  Yuanbin Li³  Naishi Li³  Yaru Wu³ 

[1] Central Laboratory, Taiyuan Central Hospital, Shanxi Medical University, Taiyuan, China;Department of Endocrinology, Shanxi DAYI Hospital, Shanxi Medical University, Taiyuan, China;Graduate School of Shanxi Medical University, Taiyuan, China

关键词: Insulin signaling;    Transcription factors;    Insulin;    Phosphorylation;    Inflammation;    Insulin resistance;    Insulin secretion;    Blood sugar;   

DOI  :  10.1371/journal.pone.0189722

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Vaspin (visceral adipose tissue-derived serine protease inhibitor) is a recently discovered adipokine that has been implicated in diabetes mellitus and other metabolic disorders. However, the effects of vaspin on pancreatic β cell function and related mechanisms are not fully understood. Thus, the present study was performed to investigate the effects of vaspin on pancreatic β cell function and the potential underlying mechanisms. Both in vitro (rat insulinoma cells, INS-1) and in vivo (high fat diet fed rats) experiments were conducted. The results showed that vaspin significantly increased INS-1 cell secretory function. Potential mechanisms were explored using inhibitors, western blot and real-time PCR techniques. We found that vaspin increased the levels of IRS-2 mRNA and IRS-2 total protein, while decreased the serine phosphorylation level of IRS-2 protein. Moreover, vaspin increased the Akt phosphorylation protein level which was reversed by PI3K inhibitor ly294002. In addition, vaspin increased the phosphorylation levels of mTOR and p70S6K, which was inhibited by rapamycin. Meanwhile, we found that the NF-κB mRNA and protein levels were reduced after vaspin treatment, similar to the effect of NF-κB inhibitor TPCK. Furthermore, vaspin increased the glucose stimulated insulin secretion (GSIS) level, lowered blood glucose level and improved the glucose tolerance and insulin sensitivity of high fat diet fed rats. Hyperglycemic clamp test manifested that vaspin improved islet β cell function. Together, these findings provide a new understanding of the function of vaspin on pancreatic β cell and suggest that it may serve as a potential agent for the prevention and treatment of type 2 diabetes.

【 授权许可】

CC BY