The Dental Plaque Microbiome in Health and Disease

PLoS One

Mogens Kilian¹  Walter Bretz²  Jia Liu³  Ana C. Ong³  Scott N. Peterson³  Erik Snesrud³  Nicholas J. Schork⁴ 

[1] Department of Biomedicine, Aarhus University, Aarhus, Denmark;Department of Cariology and Comprehensive Care, New York University, College of Dentistry, New York, New York, United States of America;J. Craig Venter Institute, Rockville, Maryland, United States of America;The Scripps Translational Science Institute and Scripps Health, La Jolla, California, United States of America

关键词: Microbiome;    Caries;    Streptococcus mutans;    Streptococcus;    Species diversity;    Campylobacter;    Neisseria;    Sequence databases;   

DOI  :  10.1371/journal.pone.0058487

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Dental decay is one of the most prevalent chronic diseases worldwide. A variety of factors, including microbial, genetic, immunological, behavioral and environmental, interact to contribute to dental caries onset and development. Previous studies focused on the microbial basis for dental caries have identified species associated with both dental health and disease. The purpose of the current study was to improve our knowledge of the microbial species involved in dental caries and health by performing a comprehensive 16S rDNA profiling of the dental plaque microbiome of both caries-free and caries-active subjects. Analysis of over 50,000 nearly full-length 16S rDNA clones allowed the identification of 1,372 operational taxonomic units (OTUs) in the dental plaque microbiome. Approximately half of the OTUs were common to both caries-free and caries-active microbiomes and present at similar abundance. The majority of differences in OTU’s reflected very low abundance phylotypes. This survey allowed us to define the population structure of the dental plaque microbiome and to identify the microbial signatures associated with dental health and disease. The deep profiling of dental plaque allowed the identification of 87 phylotypes that are over-represented in either caries-free or caries-active subjects. Among these signatures, those associated with dental health outnumbered those associated with dental caries by nearly two-fold. A comparison of this data to other published studies indicate significant heterogeneity in study outcomes and suggest that novel approaches may be required to further define the signatures of dental caries onset and progression.

【 授权许可】

CC BY