Cell Origins and Diagnostic Accuracy of Interleukin 27 in Pleural Effusions

PLoS One

Rong-Hui Du¹  Qiu-Li Liang²  Wan-Li Ma³  Huan-Zhong Shi³  Xian-Zhi Xiong³  Zhi-Jian Ye³  Chun-Mi Niu³  Jian-Chu Zhang³  Qiong Zhou³  Wei-Bing Yang³ 

[1] Department of Internal Medicine, Wuhan Institute of Tuberculosis Prevention and Control, Wuhan, China;Institute of Respiratory Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning, China;Key Laboratory of Pulmonary Diseases of Health Ministry, Department of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

关键词: Pleurae;    Pleural effusion;    T cells;    Macrophages;    Cancer detection and diagnosis;    NK cells;    Flow cytometry;    Interleukins;   

DOI  :  10.1371/journal.pone.0040450

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

The objective of the present study was to investigate the presence of interleukin (IL)-27 in pleural effusions and to evaluate the diagnostic significance of pleural IL-27. The concentrations of IL-27 were determined in pleural fluids and sera from 68 patients with tuberculous pleural effusion, 63 malignant pleural effusion, 22 infectious pleural effusion, and 21 transudative pleural effusion. Flow cytometry was used to identify which pleural cell types expressed IL-27. It was found that the concentrations of pleural IL-27 in tuberculous group were significantly higher than those in malignant, infectious, and transudative groups, respectively. Pleural CD4+ T cells, CD8+ T cells, NK cells, NKT cells, B cells, monocytes, macrophages, and mesothelial cells might be the cell sources for IL-27. IL-27 levels could be used for diagnostic purpose for tuberculous pleural effusion, with the cut off value of 1,007 ng/L, IL-27 had a sensitivity of 92.7% and specificity of 99.1% for differential diagnosing tuberculous pleural effusion from non-tuberculous pleural effusions. Therefore, compared to non-tuberculous pleural effusions, IL-27 appeared to be increased in tuberculous pleural effusion. IL-27 in pleural fluid is a sensitive and specific biomarker for the differential diagnosing tuberculous pleural effusion from pleural effusions with the other causes.

【 授权许可】

CC BY