Determination of carbohydrates in honey and milk by capillary electrophoresis in combination with graphene-cobalt microsphere hybrid paste electrodes

卷:190

Liang, Peipei ; Sun, Motao ; He, Peimin ; Zhang, Luyan ; Chen, Gang

Fudan Univ

关键词: Carbohydrate;    Capillary electrophoresis;    Amperometric detection;    Graphene;    Cobalt microsphere;    Paste electrode;   

DOI  :  10.1016/j.foodchem.2015.05.059

学科分类：食品科学和技术

【 摘 要 】

A graphene-cobalt microsphere (CoMS) hybrid paste electrode was developed for the determination of carbohydrates in honey and milk in combination with capillary electrophoresis (CE). The performance of the electrodes was demonstrated by detecting mannitol, sucrose, lactose, glucose, and fructose after CE separation. The five analytes were well separated within 9 min in a 40 cm long capillary at a separation voltage of 12 kV. The electrodes exhibited pronounced electrocatalytic activity, lower detection potentials, enhanced signal-to-noise characteristics, and higher reproducibility. The relation between peak current and analyte concentration was linear over about three orders of magnitude. The proposed method had been employed to determine lactose in bovine milk and glucose and fructose in honey with satisfactory results. Because only electroactive substances in the samples could be detected on the paste electrode, the electropherograms of both food samples were simplified to some extent. (C) 2015 Elsevier Ltd. All rights reserved.