期刊论文详细信息
卷:192
Monitoring the contents of six steroidal and phenolic endocrine disrupting chemicals in chicken, fish and aquaculture pond water samples using pre-column derivatization and dispersive liquid-liquid microextraction with the aid of experimental design methodology
Wu, Hongliang ; Li, Guoliang ; Liu, Shucheng ; Hu, Na ; Geng, Dandan ; Chen, Guang ; Sun, Zhiwei ; Zhao, Xianen ; Xia, Lian ; You, Jinmao
Qufu Normal Univ
关键词: Endocrine disrupting chemicals;    Dispersive liquid-liquid microextraction;    Pre-column derivatization;    High-performance liquid chromatography with fluorescence detection;    Box-Behnken design;   
DOI  :  10.1016/j.foodchem.2015.06.059
学科分类:食品科学和技术
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【 摘 要 】

This research established a sensitive and efficient pre-column derivatization HPLC method based on dispersive liquid-liquid microextraction (DLLME) for the simultaneous determination of six steroidal and phenolic endocrine disrupting chemicals (EDCs). In this study, EDCs were firstly labeled by the derivatization reagent 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) and then extracted by DLLME. The response surface methodology was employed to investigate the key parameters of pre-column derivatization and DLLME. Under the optimal conditions, a good linear relationship between the peak area and the concentration of analytes was observed with correlation coefficients of >0.9991. Limits of detection for all EDCs derivatives were achieved within the range of 0.02-0.07 mu g L-1. The proposed method has the advantages of simple operation, low consumption of organic solvent, saving time, low output limit and good selectivity. When applied to several food and water samples analysis, it demonstrated good applicability for the determination of EDCs. (C) 2015 Elsevier Ltd. All rights reserved.

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