Effect of complexes of cyanidin-3-diglucoside-5-glucoside with rutin and metal ions on their antioxidant activities

卷:232

Qian, Bing-Jun ; Wu, Chou-Fei ; Lu, Man-Man ; Xu, Wei ; Jing, Pu

Shanghai Jiao Tong Univ

关键词: Anthocyanins;    Cyanidin-3-diglucoside-5-glucoside;    Complexes;    Copigmentations;    Antioxidant activity;    Molecular interaction;   

DOI  :  10.1016/j.foodchem.2017.04.010

学科分类：食品科学和技术

【 摘 要 】

Studies have enabled a molecular understanding of the anthocyanin copigmentation phenomenon over several decades. However, the effect of combinations of, or even supramolecular assemblies of, anthocyanins with other phenols and/or metal ions on their antioxidative activity was unclear. In this study, anthocyanin complexes of cyanidin-3-diglucoside-5-glucoside (CY3D5G), rutin and Mg(II)/Fe(III) were constructed, analyzed, and evaluated for their antioxidant effects. The CY3D5G-rutin-Fe(III) exhibited supramolecular properties via visible, CD and FTIR spectra among complexes. The interaction of CY3D5G-rutin, CY3D5G-rutin-Mg(II), or CY3D5G-rutin-Fe(III) was synergistic (P < 0.05) in the ORAC assay. On cellular ROS levels, the median effective concentration of the CY3D5G-rutin-Mg(II) was 7.76 lmol QE/L and exhibited a synergistic interaction (CI = 0.67, P < 0.05), whereas the CY3D5G-rutin-Fe(III) (CI = 0.79, P = 0.074) was additive. The results indicate that the antioxidant properties were affected by the molecular combination. Additionally, Fe(III) might exhibit a negative effect, since the CY3D5G-Fe(III) required a greater concentration than CY3D5G to achieve the same effect on cells. (C) 2017 Elsevier Ltd. All rights reserved.